Determination of DNA in tissue sections

Date: 2015-10-07; view: 475.

Take 5-10 drops of the resulting solution of DNA, mix with the same amount of diphenylamine reagent and place in boiling water bath. Liquid gradually became blue. This reaction is caused by hydrolysis, which released deoxyribose.

Specificity of hydrogen bonds between adenine and thymine is dimensional and connection between guanine and cytosine explains Chargaff constancy in the ratio of A / T and T / D and reflects the complementary strands double helix. Specific sequence of pairs A - T and G - C can be specific and does not affect the structure of the DNA molecule, forming a double helix. Possible number of different sequences of base pairs in the DNA molecule is almost infinite, and can encode to a colossal information quantity. This fact confirms the hypothesis that DNA can serve as a matter of heredity for all organisms.

According to the model of Watson and Crick, the native DNA molecule consists of two polymer chains which are mutually connected nucleotides twisted into the form of a double helix. The bond between the chains is ensured by the special hydrogen bonds between adenine (A) and thymine (T) and between guanine (G) and cytosine (C). A is complementary to T, and G is complementary to C.

In connection with the latest achievements of science, the outstanding role of nucleic acids in protein synthesis is demonstrated. This theory links the inheritance structure of DNA.
Detection of DNA in the tissues of the spleen in vitro. Experiment
Thoroughly rub 0,5 g of spleen so100g glass powder in a porcelain mortar, gradually pouring in the amount of NaCl solution 15ml. transfer the contents of the mortar in a centrifuge tube and centrifugate. Then slowly, with stirring stick, pour into a beaker with 80-90ml of distilled water. DNA is not soluble in water; move it into a clean test tube with sodium hydroxide solution of 1-2ml to dissolve.