Physiology of microorganisms, genetics (tests for studying)

Date: 2015-10-07; view: 659.

Вывод.

Результаты измерений.

В данном опыте установлено, что ускорение свободного падения, из показаний 14 измерений, равно 9,558±0,251(м/с²).

1. Bacteriological method (pure culture isolation) is used for laboratory diagnostics of many infectious diseases. Which purpose of the first stage of investigation?

A. Seeding of the collected samples to get separated colony

B. Obtaining of the separated colonies

C. Microscopy of the investigated samples

D. Isolation and accumulation of the pure culture

E. Identification of the isolated culture

2. Bacteriological method (pure culture isolation) is used for laboratory diagnostics of many infectious diseases. Which purpose of the second stage of investigation?

A. Seeding of the collected samples

B. Obtaining of the separated colonies

C. Microscopy of the investigated samples

D. Isolation and accumulation of the pure culture

E. Identification of the isolated culture

3. Bacteriological method (pure culture isolation) is used for laboratory diagnostics of many infectious diseases. Which purpose of the third stage of investigation?

A. Seeding of the collected samples

B. Obtaining of the separated colonies

C. Microscopy of the investigated samples

D. Isolation and accumulation of the pure culture

E. Identification of the isolated culture

4. DNA-polymerase from Thermus aquaticus is important reagent of the polymerase chain reaction (PCR). This microorganism is capable of growing at 100 ⁰C and higher and it is:

A. Thermophilic

B. Mesophilic

C. Psychrophilic

D. Halophilic

E. Chemolytotrophs

5. The event, at which prophage may replicate with bacterial chromosome in the same time, is known as:

A. Transfection

B. Lysogeny

C. Transformation

D. Modification

E. Transduction

6. It is known bacterial cells include additional genetic structures, which either exists as independent element autonomically from chromosome or integrates with it, and supplements such cell additional property. What is the name of this additional elements?

A. Prophages

B. Nucleotides

C. Is-sequences

D. Plasmids

E. Migrating genes

7. The procedure of human gene incorporation into bacterial genome with bacteriophage is used in biotechnological processes for production of therapeutic and prophylactic medicines. What is the name of such process?

A. Transduction

B. Transformation

C. Translation

D. Conjugation

E. Deletion

8. A bacterial cell gets nutrients by different ways. One of them is facilitated diffusion that is realized with special membrane carrier proteins. What are they called?

1. Isomerases
2. Lyases
3. Oxydoreductases
4. Permeases
5. Ligases

9. From clinical sample a culture of bacteria ,which obtain energy in result of oxidative-reduction reactions between substrates used for nutrition, was isolated. Which group of microorganism does isolated culture belong to?

1. Chemotrophes
2. Phototrophes
3. Metatrophes
4. Organotrphes
5. Autotrophes

10. For cultivation of Brucella, pure cultures should incubated in CO₂ enriched atmosphere. What type of respiration is typical for brucella?

1. Any
2. Obligate aerobic
3. Capnophilic
4. Obligate anaerobic
5. Facultative anaerobic

11. In natural beds of sulfur amount of sulfur reaches millions tons sometimes. What type of microorganism is responsible for its arising?

A. Chemothrophs

B. Heterotrophes

C. Parasites

D. Phototrophes

E. Autotrophes

12. Acidity arises very often during growth of bacterial culture on the nutrient medium. Which process is responsible for this event?

A. Glycolisis

B. DNA replication

C. phosphate cutting off from nucleic acid

D. mineral acid formation

E. carbon dioxide formation

13.There were 2 types of colonies – red and colorless on the Endo medium after inoculation of a sample from the patient with acute enteric infection. Which group of media does this medium belong to?

1. Differential-diagnostic
2. Universal
3. Special
4. Elective

14. Diphtheria bacillus is cultivated at factory producing vaccine to get toxin. A culture medium enriched with serum is used for cultivation of this microorganism because it can not synthesize necessary for growth amino acids and vitamins itself. Which group of bacteria (due to metabolism) does it belong to?

1. Auxotrophes
2. Protothrophes
3. Litotrophes
4. Photothrophs
5. Autotrophes

15. To study sugarlytic properties of isolated pure culture it was inoculated in the test tubes with semi-solid agar that contained indicator VR and carbohydrates – glucose, lactose, mannitol, suchrose. What media were used in this case?

1. Hiss
2. Ressel
3. Loeffler
4. Endo
5. Levenstain-Jensen

16. Industrial strain of Actynomyces which is used for production of antibiotic belongs to psychrophilic microorganisms. What temperature should be used to cultivate it and create optimal condition for its growth?

1. 15-20⁰C
2. 5-10⁰C
3. 30-35⁰C
4. 36-38⁰C
5. 45-50⁰C

17. The causative agents of the intestinal infections can grow at refrigerator temperatures, which may cause infection in people. What type of temperature optimum do these microorganisms relate to?

1. Psychrophilic
2. Anthropophilic
3. Mesophilic
4. Necrophilic
5. Thermophilic

18.A patient with suspicious gas gangrene infection was admitted to hospital. Which medium should patient sample be inoculated?

A. Kitt-Tarozzi

B. Levin's

C. Endo

D. Muller's

E. Rassel's

19. A pathological sample taken from the patient with pulpitis has been seeded into the Kitt-Tarozzi medium. Which microorganisms are planned to isolate?

A. Anaerobic

B. Acid-fast

C. Acidophilic

D. Hemolytic

E. Aerobic

20.Anaerobs are the causative agents of the most part of suppurative lesions into the oral cavity. Which from the listed culture (nutrient) media may be used for control of dress material contamination with anaerobs?

a. Sabouroux

b. Roux

c. Kitt-Tarozzi

d. Ploskirev′s

e. Endo

21. After examination of a patient with necrotizing dentofacial phlegmon doctor suspected gas gangrene infection. There were gram-positive rod-shaped microorganisms in the smear under microscopy. Which medium should be used for pure culture isolation?

a. Kitt-Tarozzi

b. Levin's

c. Endo

d. Meat-peptone agar

e. Milk-salt agar

22. Multiple resistance to antibiotics has been revealed during investigation of antibioticogram for pure culture of isolated Salmonella. Which factor can be responsible for such event?

1. F-plasmids
2. Temperate phage
3. Transposons
4. R-plasmid
5. Mutation in chromosome

23. Bacteria may include both chromosomal and extrachromosomal hereditary elements – plasmids. Presence of plasmid genes may arise with:

1. Resistance to dyes
2. Multiple medicinal resistance
3. Ability of sporulation
4. Resistance to physical factors
5. Ability of motility

24. Colibacterin isused for treatment of disbiosis. This preparation includes E.coli which is capable of producing bacteriocines. What cellular structure is responsible for such ability?

1. Capsule
2. Mesosomes
3. Flagella
4. Ribosomes
5. Plasmids

25. Which enzyme is used for synthesis of different genes from matrix RNA to DNA in the gene engineering (this enzyme was revealed at RNA-including viruses)?

A. Exonucleases

B. DNA-lygases

C. Revertases

D. Endonucleases

E. Chelicases

26.The event, at which prophage may replicate with bacterial chromosome in the same time, is known as:

A. Transfection

B. Lysogeny

C. Transformation

D. Modification

E. Transduction

27. It is known bacterial cells include additional genetic structures, which either exists as independent element autonomically from chromosome or integrates with it, and supplements such cell additional property. What is the name of this additional elements?

A. Prophages

B. Nucleotides

C. Is-sequences

D. Plasmids

E. Migrating genes

28.The procedure of human gene incorporation into bacterial genome with bacteriophage is used in biotechnological processes for production of therapeutic and prophylactic medicines. What is the name of such process?

A. Transduction

B. Transformation

C. Translation

D. Conjugation

E. Deletion