Methodical instructions

Date: 2015-10-07; view: 482.

M.O. Auezov South-Kazakhstan State University

MINISTRY OF THE EDUCATION AND SCIENCE OF THE REPUBLIC OF KAZAKHSTAN

Department: “Biotechnology”

B.Zh. Mutaliyeva, K.Shoinbaeva

General and molecular genetics

to laboratory classes on the discipline of “General and molecular genetics” for students

of the 050701 “Biotechnology” speciality

Training forms: full-time

UDC 577.1

BBK

Compilers: B.Zh. Mutaliyeva, K.Shoinbaeva

Methodical instructions to laboratory classes on the discipline of “General and molecular genetics” – Shymkent: M. Auezov SKSU. - 2010. - 40 p.

Methodical instructions was composed in accordance to requirements of the curriculum and “General and molecular genetics” discipline programme and include all nessesary informations on the given course.

Methodical instructions is intended for the students of the 050701 “Biotechnology” specialty. Methodical instructions help the students to check and master the knowledges. The course consist of 8 laboratory classes including different experimantal materials for mastering of themes in accordance with State obligatory education standard of the RK.

Reviewer: Narymbayeva Z.K.– ch.s.c., p.d. of the associated professor of the M. Auezov SKSU

Madybekova G.M. – ch.s.c., associated professor of the Chemistry department of the Academical innovation university

Considered and recommended by meeting of “Biotechnology” department for printing (record № 1 from 28. 08. 2010 y.),

Methodical Advice of the Agroindustrial faculty (record №_1__from 29.08.2010 y.)

Recommended for printing by Educational-Methodical Advice of the M. Auezov SKSU (record №___ from “___” ____2010 y.)

© South-Kazakhstan State University, 2010 y.

Responsible for issue -

CONTENTS

Introduction
Safety instructions during the work in the laboratory.

Laboratory work №1. Subject and tasks of genetics. Acquaintance with the objectives of laboratory work, objects and methods of work, the reporting system. Acquaintance with the subject of laboratory research: wheat, Drosophila melanogaster.
Laboratory work №2. Genetic analysis. Hybridological method - the interbreeding method. Genetical symbols, rules for writing crosses. Types of crosses used in genetics. Analyzing cross.
Laboratory work №3. Rules of experiment execution: the management of experiments protocol, preparation of a working hypothesis, interbreeding scheme, the construction of the conclusions. Working with the fruit fly - the main object of the laws of inheritance and variability of symptoms. Of experiments with mono-and dihybrid interbreeding.
Laboratory work №4. Analysis of the first generation of monohybrid and dihybrid interbreeding on Drosophila, construction of experiments for producing of F2. Seminar of discussing the theory, dihybrid and polyhybrid interbreeding.
Laboratory work №5. Analysis of the F2. Statistical processing of results using mathematical method. Getting the skills of writing a report on the results of studies.
Laboratory work №6. Analysis of F1 in the experiment number 3 and the staging of crosses to produce second generation (experiment number 3). Genetics of sex. Mechanisms ensuring gender balance in nature. Inheritance of sex-linked features.
Laboratory work №7. Linked inheritance and crossingover. General statements of the inheritance chromosome theory. Genetical and cytological evidence of crossingover. The mechanism of crossingover. Unequal crossing over. Mitotic crossingover.
Laboratory work №8. Variability of genetic material on the basis of genes, chromosomes, genomes. Physical, chemical, biological and environmental factors and their effects. The problems of genetic safety. Methods of qualitative and quantitative assessment of mutations. The solution of genetic problems.
Laboratory work №9. The structure of DNA and RNA. Purines and pyrimidines. Ribose and deoxyribose. Phosphoric acid residues.Structure of nucleotide monomers of nucleic acids. Types of nucleic acids. The chemical structure of nucleic acids. Primary and three-dimensional structure of DNA. Forms of messenger RNA (mRNA, rRNA, tRNA).
References